Skip to main content

Table 5 All bacteria real-time PCR detection with a universal system in 41 diarrheal stool specimens, before and after lyophilization (Ct value)

From: Optimized microbial DNA extraction from diarrheic stools

Sample n°

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

Without lyophilization

NA

NA

30.54

NA

28.83

34.47

35.95

36.64

27.85

28.04

34.85

34.02

NA

30.14

26.82

With lyophilization

34.65

17.44

26.86

29.49

28.21

24.99

23.43

39.07

22.81

25.02

22.53

32.33

25.78

28.33

26.36

Sample n°

31

32

33

34

35

36

37

38

39

40

41

42

43

44

45

Without lyophilization

33.42

26.45

34.22

NA

34.63

30.36

28.49

26.98

33.14

23.75

NA

22.79

31.95

31.05

33.50

With lyophilization

21.18

22.03

21.79

26.01

21.84

24.4

29.74

23.24

28.85

21.29

33.43

19.31

21.41

32.68

22.23

Sample n°

46

47

48

49

50

51

52

53

54

55

56

    

Without lyophilization

29.24

NA

NA

24

23.21

23.05

17.59

19.94

14.65

18.33

18.58

    

With lyophilization

20.62

35.95

43.68

22.91

10.82

23.24

16.33

19.66

14.43

19.15

19

    
  1. NA, not amplified. The proportion of positive specimens was significantly higher after lyophilization for the detection of Bacteria (p = 0.015).