CD4+ CD25+CD127low surface staining. CD4+CD25+ regulatory T cells are known to downregulate CD127 antigen cells surface. This is correlate with FoxP3 production. Present gating strategy allows us to determine proportion of CD25+CD127 Low/- in our study subject's samples. a) Lymphocytes determination lead us to CD4 T lymphocytes (b) in white cells we to CD25+CD127low subsets cells (c).