A multi-laboratory profile of Mycoplasma contamination in Lawsonia intracellularis cultures

BMC Research Notes

Table 1 PCR primer sequences

Target gene	Primer	Nucleotide sequence (5'→3')^a	Reference
16S rRNA	MyU 5-1	CGCCTGAGTAGTACGTTCGC	[26]
	MyU 5-2	CGCCTGAGTAGTACGTACGC
	MyU 5-3	TGCCTGAGTAGTACATTCGC
	MyU 5-4	TGCCTGGGTAGTACATTCGC
	MyU 5-5	CGCCTGGGTAGTACATTCGC
	MyU 5-6	CGCCTGAGTAGTATGCTCGC
	MyU 3-1^b	GCGGTGTGTACAAGACCCGA
	MyU 3-2^b	GCGGTGTGTACAAAACCCGA
	MyU 3-3^b	GCGGTGTGTACAAACCCCGA
16S and 23S rRNA	F1 (OF)	ACACCATGGGAGYTGGTAAT	[27]
	R1^b (OF)	CTTCWTCGACTTYCAGACCCAAGGCAT
	FN2 (IF)	ACCTCCTTTCTACGGAGTACAA
	R2^b (IR	GCATCCACCAWAWACYCTT
	FN3 (IF)	TATTTGCTATTCAGTTTTCAAAGAAC
	RN3^b (IR)	GGGGTGAAGTCGTAACAAGGTAT

^a Abbreviations represent a mixed-base code, which was used to reduce the number of primers. Y = C, T and W = A, T
^b Primers are in the reverse orientation

ISSN: 1756-0500