Figure 4
Confirmation of the expression of two novel miRNAs, miR-101 and miR-16, in monkey (marmoset), rabbit and dolphin. PCR was used to amplify expressed miRNAs based on presence in miRviewer. Amplified fragments were stained and separated in Agarose gel then excised and sequenced for confirmation (Additional file 1: Figure S1). These miRNAs were identified as part of a very large set of homologous miRNAs using the miRNAminer tool [7] all of which are presented in miRviewer.