Effect of the isolation procedure on the surface expression of CD14 on neutrophils. Whole blood or isolated neutrophil was stained with CD14 mAb. Blood was anticoagulated with HEPARIN. Using flow cytometry, the percentage of PMN expressing mCD14 (3A) and the mean channel fluorescence intensity (MFI) (3B) was measured. Results for each treatment are the mean from 5 cows. Treatment means with different letters are significantly different (p < 0.05) using Scheffe's multiple comparison tests.