Figure 4

Effect of the SQDG-defect on the cytogram of Chl fluorescence vs. cell size or DNA content vs. cell size. Wild-type cells were normally cultured whereas SD1 cells precultured with supplementation of SQDG were transferred to SQDG-replete or -deprived conditions for 3 days. In individual cells of the wild type or SD1, Chl fluorescence vs. forward light scattering (A-C), or DNA content vs. forward light scattering (D-F) was measured with a flow cytometer, as described in Materials and methods. The results were visualized in 2D color density plot such that blue-to-red color changes in the plot indicate dot-density changes from low to high. Shown are the results representative of three independent experiments. (A), (D): Wild-type cells. (B), (E): SD1 cells replete of SQDG. (C), (F): SD1 cells defective in SQDG.