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Figure 3 | BMC Research Notes

Figure 3

From: Effect of shear stress and substrate on endothelial DAPK expression, caspase activity, and apoptosis

Figure 3

Shear stress on cells plated on membrane induced apoptosis similar to cells on glass. (A) TUNEL positive cells are presented as fold increase over static control on either substrate. Sheared cells demonstrated significant higher apoptosis compared to static control cells on both glass and membrane substrate. However, sheared cells on membrane cells exhibited significantly less apoptosis when compared to the sheared cell on glass. Data represent average ± standard error for n ≥ 4. * P < 0.01 compared to control-glass cells, + P < 0.01 compared to control-membrane cells, and # P < 0.01 compared to 6hr shear-glass. (B) Caspase-3 and −7 results are presented as fold increase over static control BAEC, and shear stress also induced significantly increased caspase 3/7 activities compared to static control cells on both substrates. Again, sheared cells on membrane exhibits significantly decreased caspase activity compared to sheared cells on glass. Data represent average ± standard error for n ≥ 5. * P < 0.01 compared to control-glass cells, + P < 0.05 compared to control-membrane cells, and # P < 0.05 compared to 6 hr shear-glass.

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