Skip to main content

Table 2 Primers used in multiplex PCR for SCC mec typing

From: Sccmec type II gene is common among clinical isolates of methicillin-resistant Staphylococcus aureus in Jakarta, Indonesia

Locus

Name

Oligonecleotide sequence (5’-3)

Amplicon size (bp)

Specificity ( SCCmec type)

A

CIF2 F2

TTCGAGTTGCTGATGAAGAAGG

495

I

CIF2 R2

ATTTACCACAAGGACTACCAGC

B

KDP F1

AATCATCTGCCATTGGTGATGC

284

II

KDP R1

CGAATGAAGTGAAAGAAAGTGG

C

MECI P2

ATCAAGACTTGCATTCAGGC

209

II, III

MECI P3

GCGGTTTCAATTCACTTGTC

D

DCS F2

CATCCTATGATAGCTTGGTC

342

I, II, IV

DCS R1

CTAAATCATAGCCATGACCG

E

RIF4 F3

GTGATTGTTCGAGATATGTGG

243

III

RIF4 R9

CGCTTTATCTGTATCTATCGC

F

RIF5 F10

TTCTTAAGTACACGCTGAATCG

414

III

RIF5 R13

GTCACAGTAATTCCATCAATGC

G

IS431 P4

CAGGTCTCTTCAGATCTACG

381

pUB11C

Pub110 R1

GAGCCATAAACACCAATAGCC

H

IS431 P4

CAGGTCTCTTCAGATCTACG

303

III

pT181 R1

GAAGAATGGGGAAAGCTTAC

mecA

MECA P4

TCCAGATTACAACTTCACCAGG

162

Positive control

 

MECA P7

CCACTTCATATGTTGTAGG