Figure 3From: Lung tissue bioenergetics and caspase activity in rodentsWistar rat lung tissue respiration, ATP content and caspase activity with and without 30 min prior incubation with glucose oxidase. Lung fragments were incubated at 37°C in 2.0 mL MEM with and without 200 μg glucose oxidase (which totally depleted O2 from the solution) for 30 min. The samples were then rinsed with MEM and incubated at 37°C in 50 mL MEM (continuously gassed with 95% O2: 5% CO2) for up to ~7 h. At indicated time periods, samples were removed from the incubation medium and processed for measurements of O2 consumption, ATP content and caspase activity. Panel A: Runs of cellular mitochondrial O2 consumption are shown; t = 0 corresponds to animal sacrifice. The values of k c (μM O2 min-1 mg-1) are shown at the bottom of each run. Panel B: The values of k c and ATP are plotted as a function of incubation time. Panel C: HPLC runs of caspase activity at 0, 3 and 6 h of incubation, showing only the AMC peak at ~4.8 min (solid lines, with GO; dotted lines, with GO). The substrate run (dashed line) was sample incubated without lung specimen for 6 h. Panel D: AMC peak areas are shown as a function of incubation period.Back to article page