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Figure 7 | BMC Research Notes

Figure 7

From: Lung tissue bioenergetics and caspase activity in rodents

Figure 7

Lung tissue caspase-3 immunostaining. Lung fragments from a Wistar rat were incubated at 37°C in 50 mL MEM continuously gassed with 95% O2:5% CO2 (Panels B–C) or kept without gassing (Panels E–F). The tissues were then stained with rabbit anti-cleaved caspase-3 antibody at 0, 3, and 6 h. For Panel A–D, the lung specimen was fixed immediately after tissue collection. Please note that the H&E (Figure 6) and caspase-3 immunostain (Figure 7) pictures correspond to the same areas. There was an increase in percentage of cells in apoptosis with time (brown staining cells) in both experimental conditions. (Immunoperoxidase, 40x).

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