Figure 1

Denaturing agarose gel electrophoresis of total RNA isolated from A. indica and other plant tissues using modified CTAB-based methods. (A) Quality of total RNA isolated by using modified extraction buffer containing only PVPP and precipitation done by using glacial acetic acid. (B) RNA isolated by using modified extraction buffer containing only activated charcoal and precipitation done by using glacial acetic acid. (C) RNA extracted by using modified extraction buffer containing activated charcoal as well as PVPP followed by glacial acetic acid precipitation. (D) RNA extracted by using modified extraction buffer containing activated charcoal as well as PVPP followed by 2.5 M Lithium Chloride precipitation. (E, F) RNA extracted from different tissues of Azadirachta indica and other medicinal and aromatic plants by using our modified and optimized protocol.