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Table 1 Purity and yield of total RNA extracted from different plant tissues using our modified RNA extraction procedure

From: Activated charcoal-mediated RNA extraction method for Azadirachta indica and plants highly rich in polyphenolics, polysaccharides and other complex secondary compounds

S. no.

Family

Plant species

Tissue

Purity (A260/280)

Purity (A260/230)

Total yield (μg/g tissue)

1

Meliaceae

Azadirachta indica

Immature fruit

1.87 ± 0.02

2.32 ± 0.15

112 ± 25

  

Azadirachta indica

Mature fruit

1.99 ± 0.08

1.81 ± 0.03

341 ± 56

  

Azadirachta indica

Young leaf

1.92 ± 0.04

2.25 ± 0.07

354 ± 36

  

Azadirachta indica

Mature leaf

1.90 ± 0.09

1.77 ± 0.07

107 ± 70

  

Azadirachta indica

Fruit endocarp

2.04 ± 0.07

2.15 ± 0.06

234 ± 31

  

Azadirachta indica

Fruit mesocarp

2.02 ± 0.16

2.06 ± 0.03

45 ± 15

2

Phyllanthaceae

Phyllanthus amarus

Leaf

1.91 ± 0.06

2.24 ± 0.09

288 ± 21

3

Xanthorrhoeaceae

Aloe vera

Leaf

1.86 ± 0.05

2.14 ± 0.03

10 ± 1

4

Geraniaceae

Pelargonium graveolens

Leaf

1.80 ± 0.09

1.67 ± 0.06

100 ± 22

5

Rosaceae

Rosa damascena

Buds

1.79 ± 0.17

1.53 ± 0.63

14 ± 4

  

Rosa damascena

Flowers

1.84 ± 0.14

1.75 ± 0.16

28 ± 11

6

Acanthaceae

Andrographis paniculata

Leaf

2.01 ± 0.11

1.87 ± 0.05

122 ± 18

7

Asteraceae

Artemisia annua

Leaf

1.95 ± 0.02

2.13 ± 0.10

213 ± 20

  

Wedelia chinensis

Leaf

1.96 ± 0.07

2.36 ± 0.06

181 ± 58

8

Fabaceae

Tephrosia purpurea

Leaf

1.94 ± 0.04

2.26 ± 0.22

216 ± 33

  

Glycyrrhiza glabra

Leaf

1.95 ± 0.16

2.44 ± 0.21

70 ± 27

9

Convolvulaceae

Merremia gangetica

Leaf

2.02 ± 0.17

2.43 ± 0.05

80 ± 7

10

Lamiaceae

Ocimum basilicum

Leaf

1.93 ± 0.04

1.97 ± 0.03

256 ± 33

  

Ocimum gratissimum

Leaf

1.84 ± 0.02

1.92 ± 0.01

302 ± 33

  

Ocimum sanctum

Leaf

1.86 ± 0.03

2.05 ± 0.06

203 ± 55

  

Mentha arvensis

Leaf

1.93 ± 0.01

2.37 ± 0.10

177 ± 23

  

Mentha piperita

Leaf

1.82 ± 0.13

2.20 ± 0.24

219 ± 53

11

Apocynaceae

Catharanthus roseus

Leaf

1.87 ± 0.10

2.22 ± 0.09

262 ± 23

  

Hemidesmus indicus

Leaf

1.95 ± 0.12

2.46 ± 0.07

105 ± 35

12

Solanaceae

Withania somnifera

Leaf

1.92 ± 0.10

2.31 ± 0.10

291 ± 25

13

Plantaginaceae

Scoparia dulcis

Leaf

1.88 ± 0.09

2.14 ± 0.13

214 ± 28

  

Bacopa monnieri

Leaf

1.94 ± 0.08

2.35 ± 0.25

90 ± 14

14

Cannabaceae

Cannabis sativa

Leaf

2.06 ± 0.06

2.28 ± 0.05

194 ± 64

  1. The RNA yield was calculated based on the following formula: yield = A 260 × 40 × 1,000 × volume (μl)/material weight (g). Each value indicates the mean of 3 (n = 3) replicates and standard error (±SE). The statistical analysis was performed by SPSS 11.5 software.
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BMC Research Notes

ISSN: 1756-0500

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