Germ-line DICER1 mutations do not make a major contribution to the etiology of familial testicular germ cell tumours

Sabbaghian, Nelly; Bahubeshi, Amin; Shuen, Andrew Y; Kanetsky, Peter A; Tischkowitz, Marc D; Nathanson, Katherine L; Foulkes, William D

doi:10.1186/1756-0500-6-127

Table 2 DICER1 oligonucleotide primers used in this study

From: Germ-line DICER1 mutations do not make a major contribution to the etiology of familial testicular germ cell tumours

Exon	Forward	Reverse	Size of fragment	Annealing temp. Sanger sequencing	Annealing temp. HRM
2	GCAATGAAAGAAACACTGGATG	TCAAATCCAATTACCCAGCAG	358	[1]	64
3	TTTTGTAAATTTATTGGAGGACG	TCTGCCAGAAGAGATTAAATGAG	429	[1]	64
4	TTTTGGAGGATAACCTTGGAAC	AAATCAGACAACCAAGGCTACAG	390	[1]	66
5	TTGTCGTCAAGACATGCTTTC	TTTAATATTCATTCATTCATACACTGC	518	[1]	66
6	TAGTGGCATTTCCACCAAAC	GAATTCTTACTCTTGCCCATTCC	437	[1]	69
7	TTCTCACTACTGCAGTATTGATACCTT	GAGCCGCATTAAGCATATTTTC	303	[1] (7 F modified)	69
8	AAATCCCAGTTAAACCCCAC	TCACATCACAACACAGGACG	554	[1]	68
9	TAAATCACCGTCGCCAAATC	AAATCACTCTACAGCTACCTCATGG	591	[1]	69
10	CATGTGTGTCAGAAATGACAGTTG	TTCCTATGGATACAAAGAATAACAAAG	431	[1]	68
11	AGCAGGTTACTTTGGAGTACTGAAG	AACTTTTATTGCTGCACGATACTG	498	[1]	69
12	TCACATTTCAAGTGCTCACC	TGAACATGTAGATGACTACAAAAGC	596	[1]	69
13	TTTTACTAGGCAGGACTTTTAAAGATG	AAGTGTTCATGGTGCATGATTC	585	[1]	NA
14	TTTGCAGTCCAGCTCATATTG	AAGCTGTGAATCGGAGAAAG	498	[1]	69
15	TAAGAAGTGTCATGCCTCGG	TCTAGTGGAGAAATAGAAGAGGCAC	468	[1]	68
16	GAAAGCATCATTTCTGTTCTGAAG	AAGAGAAAAACGACTCTTTAGC	443	16R is new	65
17	TTCAGCATACTGTGTTCTACCTCTT	TTTTAGTAGAGACGAGGTTTCACC	484	17 F is new	69
18	TGTAAAGGTGCCATTTAGCTTC	TTTGTGTGCAAAGCATCTCC	589	[1]	69
19	ATTGCACTTGAGGGATTCTTACC	TTTGTGATATATTAATGGGCCAAG	496	[1]	67
20	TTGGCCCATTAATATATCACA	TCTCACTCCAACTGTTATGGCTTA	594	[1]	NA
21-1	AATTGCTGTTGCTCTCAGCC	GAGTACATTCATCGCTGGGC	508	[1]	68
21-2	ACAAGCAGGAAATACCCGTG	ACTGCAAACCACTTTCAGGC	501	[1]	68
22	AAAGCATAGAATATGTGGGAATT	AGAAATTTGCCTCCATCAAA	584	[1]	NA
23-1	AACCCTTGCTTTTATTGAGTTTC	CAGGGCTTCCACACAGTCC	574	[1]	NA
23-2	AAACTGTGGTGTTGACACGG	TACAAGGCCAACACGATGAG	571	[1]	68
24	TGTGGGGATAGTGTAAATGCTTC	TGCCGTCAGAACTCTGAAAC	403	[1]	68
25-26	TGGACTGCCTGTAAAAGTGG	TGAACTTTTCCCCTTTGATG	450	[1]	66
27	CCTGTCTGTCGGGGGTATG	TCTGCCTTCAATTCATTCCA	448	[1]	69

Key: HRM- High Resolution Melt. Oligonucleotide primers 16R and 17 F are new; they were designed specifically for High Resolution Melt analysis.
NA: Not Applicable – the fragments amplified were not included in the HRM assay, they were sequenced instead.
[1] refers to the source of the oligonucleotide primer sequences. bp - base pairs.

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ISSN: 1756-0500

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