Table 4 Primer pairs used for sequencing the mitogenome of Lophelia pertusa
Forward primer | Reverse primer | Annealing T° | Fragment length | Reference |
|---|---|---|---|---|
5′-AAATCAAACGAGATTCCGAGAG-3′ | 5′-TCCATGGGGACTTCTCGTC-3′ | 53°C | 1198 bp | this article |
5′-TCGACTGTTTACCAAAAACATAGC-3′ | 5′-AAYAACCTTCCATTGCATCC-3′ | 53°C | 1519 bp | this article |
5′-TAGGAGTGGTTGGGAAATCG-3′ | 5′-CTTGGGGAAGCCAAATATGA-3′ | 53°C | 2563 bp | this article |
5′-GAACAACAGGGGCAACAGAT-3′ | 5′-ATGGTGTCCCTGAAAAGTCG-3′ | 53°C | 2127 bp | this article |
5′-GCAGACGCGGTGAAACTTA-3′ | 5′-TACCCCGGCTAAGACAACTG-3′ | 53°C | 2551 bp | this article |
5′-TTGTGGGGCAAATCATTCTT-3′ | 5′-AATGAGAAAGCCCACAAGCA-3′ | 53°C | 1034 bp | this article |
5′-CAACTCCGGTTTCTGCCTTA-3′ | 5′-TTTAAAAGAAAACTATGGAGGCCTAA-3′ | 53°C | 3060 bp | this article |
5′-TTATTGGGCCTGTGTTTGGT-3′ | 5′-CCCACATATGAAAAGGAGCAAC-3′ | 53°C | 1604 bp | this article |
5′-TGGGTGCTCTTTCTTCTGGT-3′ | 5′-AAATCCAATTGGTATATAATTTGTCA-3′ | 53°C | 1237 bp | this article |
5′-ATCCCTCCTTTTGCAGGATT-3′ | 5′-CCCCAGAAGCTGTTGTGTTT-3′ | 53°C | 868 bp | this article |
5′-GGCAATTGGTTCTGGGATAA-3′ | 5′-AAGCATACTAAAAGCCGTTCCA-3′ | 53°C | 1254 bp | this article |
5′-GGTCAACAAATCATAAAGATATTGG-3′ | 5′-TAAACTTCAGGGTGACCAAAAAATCA-3′ | 45°C | 709 bp | [58] |
5′-GCCGGTGCTATTACAATGCT-3′ | 5′-CAATCGATTCAAGCTCTTTTCA-3′ | 53°C | 1892 bp | this article |