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Table 5 Primer pairs used for phylogeographic analysis in Lophelia pertusa

From: Lophelia pertusa corals from the Ionian and Barents seas share identical nuclear ITS2 and near-identical mitochondrial genome sequences

Marker

Forward primer

Reverse primer

Annealing T°

Fragment length

Reference

ITS2 (nuclear)

5′-AGCCAGCTGCGATAAGTAGTG-3′

5′-GCTGCAATCCCAAACAACCC-3′

53°C

603 bp

[64]

CR (mitochondrial)

5′-AGGGGCCTTGTTCAATTTCT-3′

5′-AGGGAGAGGGCAAATTCACT-3′

53°C

941 bp

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