Skip to main content

Advertisement

Figure 2 | BMC Research Notes

Figure 2

From: Identifying disruptors of male germ cell development by small molecule screening in ex vivo gonad cultures

Figure 2

Examples of gating strategy for flow cytometric analysis of fetal germ cells and gonadal somatic cells based on a PBS treated control sample. A. Side vs forward scatter plot showing gate P1 around cells and excluding cell debris. B. Propidium iodide in the PE-Cy5 width vs propidium iodide in the PE-Cy5 area showing events from P1, with single cells gated within P2. C. MVH-alexa fluor 488 vs propidium iodide area showing events from P2, with germ cells (MVH positive) gated in P3 and somatic (MVH negative) cells gated in P4. D. EdU-Click-iT 647 in the APC-area channel vs propidium iodide area showing events from P3 (ie germ cells), with proliferating (EdU positive) cells gated in P5. E. EdU-Click-iT 647 in the APC-area channel vs propidium iodide area showing events from P4 (ie somatic cells), with proliferating (EdU positive) cells gated in P6.

Back to article page