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Table 1 Flow cytometry controls

From: Identifying disruptors of male germ cell development by small molecule screening in ex vivo gonad cultures

Control sample EdU Stain Purpose
1. Mesonephroi × 4 No • Click-iT 647 Negative sample for establishing gates in the 647 nm and 488 nm channels
• MVH-alexa fluor 488
• Propidium iodide
2. Gonads × 4 Yes • Click-iT 647 Positive controls for setting the laser power in the 488 nm and 647 nm channels. Use females gonads for this if concentrating of male germ cell development and vice-versa if concentrating on female germ cell development (or a mix, if concentrating on both).
• MVH-alexa fluor 488
• Propidium iodide
3. Mesonephroi × 2 (optional) Yes • Click-iT 647 only Single color control for staining compensation
4. Mesonephroi × 2 (optional) No • Propidium iodide only Single color control for staining compensation
5. Gonads × 2 (optional) No • MVH-alexa fluor 488 only Single color control for staining compensation