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Table 1 CD4 T cell counting in absolute cells/μl and percentage in 182 HIV-1-infected adults and 76 HIV-1-infected children older than 5 years, by the Apogee Auto40 flow cytometer at the hôpital Militaire D’Instruction, N’Djamena, Chad, and by the FACSCalibur at the hôpital de la Liberté, N’Djamena

From: Single-platform, volumetric, CD45-assisted pan-leucogating flow cytometry for CD4 T lymphocytes monitoring of HIV infection according to the WHO recommendations for resource-constrained settings

Categories   Adults Children
Number   N = 182 N = 76
Absolute CD4 T cells (cells/ μl)    
Apogee Auto40 Mean ± SD 627 ± 591 1432 ± 821
FACSCalibur Mean ± SD 635 ± 588 1449 ± 835
Absolute bias (limits of agreement)a Mean (±1.96 SD) −16.0 (−38.2;6.1) 30.0 (−14.7;88.7)
Relative (%) bias (limits of agreement)b Mean (±1.96 SD) 1.0 (−1.8;3.8) 0.3 (−2.2;2.7)
Percentage of CD4 T cells (%CD4)    
Apogee Auto40 Mean ± SD 29.1 ± 12.8 32.1 ± 10.1
FACSCalibur Mean ± SD 28.5 ± 12.5 32.3 ± 10.6
Absolute bias (limits of agreement)a Mean (±1.96 SD) −3.0 (−7.1;1.1) 1.0 (−0.4;2.4)
Relative (%) bias (limits of agreement)b Mean (±1.96 SD) −11.0 (−25.8;3.8) 3.0 (−1.3;5.3)
  1. a The Bland Altman analysis was carried out to calculate the absolute bias and limits of agreement which are the 95% confidence intervals (±1.96 × SD) of the mean bias of all paired measurements in a given category [27];
  2. b The Pollock analysis was carried out to calculate the relative bias and limits of agreement which are the 95% confidence intervals (±1.96 × SD) of the mean bias of all paired measurements in a given category [28].
  3. SD: Standard deviation.