|
Categories
| |
< 200 cells/μl
|
200 – 350 cells/μl
|
> 350 cells/μl
|
|---|
|
Number
| |
N = 27
|
N = 43
|
N = 188
|
|---|
|
Absolute CD4 T cells (cells/
μl)*
| | | | |
|
Apogee Auto40
|
Mean ± SD
|
118 ± 161
|
278 ± 129
|
2990 ± 3745
|
|
FACSCalibur
|
Mean ± SD
|
100 ± 135
|
215 ± 103
|
3017 ± 3756
|
|
Absolute bias (limits of agreement)a
|
Mean (±1.96 SD)
|
−4.0 (−33.8;+25.8)
|
10.0 (−70.0;+90.0)
|
12.0 (−160.7;+184.7)
|
|
Relative (%) bias (limits of agreement)b
|
Mean (±1.96 SD)
|
62.0 (−134.0;+258.0)
|
7.0 (−27.2;+41.2)
|
−3.0 (−12.4;+6.4)
|
|
Percent CD4 T cells (%CD4)**
| | | | |
|
Apogee Auto40
|
Mean ± SD
|
10.0 ± 12.7
|
19.5 ± 6.3
|
55.1 ± 44.5
|
|
FACSCalibur
|
Mean ± SD
|
9.0 ± 11.3
|
18.0 ± 4.2
|
53.3 ± 41.7
|
|
Absolute bias (limits of agreement)a
|
Mean (±1.96 SD)
|
1.0 (−1.8;+3.8)
|
2.0 (−2.3;+6.3)
|
2.0 (−3.4;+7.4)
|
|
Relative (%) bias (limits of agreement)b
|
Mean (±1.96 SD)
|
6.0 (−10.3;+22.3)
|
7.0 (−13.2;+27.2)
|
2.0 (−4.6;+8.6)
|
- * Results in absolute CD4 T cell counts by Auto40 and FACSCalibur were correlated using the non-parametric Passing–Bablok regression analysis (< 200 cells/μl: r = 0.86, slope = 1.11, intercept = −12.0; 200 – 350 cells/μl: r = 0.68, slope = 1.31, intercept = −96.5; > 350 cells/μl: r = 0.98, slope = 1.02, intercept = −24.8);
- ** Results in percentage CD4 T cell counts by Auto40 and FACSCalibur were correlated using the non-parametric Passing–Bablok regression analysis (< 200 cells/μl: r = 0.89, slope = 1.00, intercept = 0.0; 200 – 350 cells/μl: r = 0.89, slope = 1.00, intercept = 0.0; > 350 cells/μl: r = 0.95, slope = 1.00, intercept = 0.0);
- a The Bland Altman analysis was carried out to calculate the absolute bias and limits of agreement which are the 95% confidence intervals (±1.96 × SD) of the mean bias of all paired measurements in a given category [27];
- b The Pollock analysis was carried out to calculate the relative bias and limits of agreement which are the 95% confidence intervals (±1.96 × SD) of the mean bias of all paired measurements in a given category [28].
- SD: Standard deviation.
