Figure 4

Manually curated variation network between the wild-type and the succinate producing mutant described in Table1. Wild-type exclusive reactions are shown in red, the ones exclusive to the mutant are in blue, while green and orange show reactions with increased or reduced flux in the mutant, respectively. Abbreviations: M_10fthf_c – 10-Formyltetrahydrofolate, M_6pgc_c- 6-Phospho-D-gluconate, M_6pgl_c- 6-phospho-D-glucono-1,5-lactone, M_f6p_c- D-Fructose-6-phosphate, M_fad_c- Flavin adenine dinucleotide oxidized, M_fadh2_c- Flavin adenine dinucleotide reduced, M_for_c- Formate, M_g6p_c- D-Glucose-6-phosphate, M_gly_c- Glycine, M_mlthf_c- 5,10-Methylenetetrahydrofolate, M_nadp_c- Nicotinamide adenine dinucleotide phosphate, M_nadph_c- Nicotinamide adenine dinucleotide phosphate reduced, M_r5p_c- alpha –D-Ribose-5-phosphate, M_ru5p_D_c- D-Ribulose-5-phosphate, M_s7p_c- Sedoheptulose-7-phosphate, M_ser_L_c- L-Serine, M_succ_c- Succinate, M_thf_c- 5, 6, 7, 8-Tetrahydrofolate, M_xu5p_D_c- D-Xylulose-5-phosphate; R_FTHFD- formyltetrahydrofolate deformylase, R_G6PDH2r- glucose 6-phosphate-1-dehydrogenase, R_GHMT2- Serine hydroxymethyltransferase, R_GND- 6-phosphogluconate dehydrogenase, R_PGI- phosphoglucose isomerase, R_PGL- 6-phosphogluconolactonase, R_SSALy- succinate-semialdehyde dehydrogenase, R_SUCCt2b- succinate transporter, R_SUCD1i- succinate dehydrogenase, R_TKT1- transketolase.