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Table 4 Projection of the range of HCECs obtainable each passage at confluent

From: Optimization of human corneal endothelial cell culture: density dependency of successful cultures in vitro

Passage no. Projected HCECs at each passage
P0 8.0 × 105 to 1.0 × 106 cells
P1 2.1 × 106 to 3.2 × 106 cells
P2 4.5 × 106 to 7.5 × 106 cells
P3 1.0 × 107 to 2.5 × 107 cells
  1. Based on the isolation protocol, the cultivation methodology described in this paper, and a seeding density of not less than 1.0 × 104 cells per cm2, it is possible to scale up the number of HCECs to a range between 1.0 × 107 to 2.5 × 107 cells at confluence, by the third passage from a pair of donor corneas.