Passage no.
|
Projected HCECs at each passage
|
---|
P0
|
8.0 × 105 to 1.0 × 106 cells
|
P1
|
2.1 × 106 to 3.2 × 106 cells
|
P2
|
4.5 × 106 to 7.5 × 106 cells
|
P3
|
1.0 × 107 to 2.5 × 107 cells
|
- Based on the isolation protocol, the cultivation methodology described in this paper, and a seeding density of not less than 1.0 × 104 cells per cm2, it is possible to scale up the number of HCECs to a range between 1.0 × 107 to 2.5 × 107 cells at confluence, by the third passage from a pair of donor corneas.