Figure 1
From: A novel pair of inducible expression vectors for use in Methylobacterium extorquens
Map of expression vectors and sequences of P R/cmtO and P R/tetO . (A) Vectors maps of pLC290 (cumate inducible) and pLC291 (aTc inducible) expression vectors. The multiple cloning site contains a variety of common, single-cutting restriction sites. oriT: RP4/RK2 transfer origin. t rrnB : transcriptional terminator. trfA, oriV: RK2 (IncP) replication protein and origin. ColE1: High-copy replication origin for E. coli (B) Sequences for used for the P R/cmtO and P R/tetO hybrid promoters. Known RNA polymerase interaction sites (bold text) and engineered operators (underlined) are indicated.