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Table 1 Growth substrate dependence on P R/cmtO and P R/tetO activation

From: A novel pair of inducible expression vectors for use in Methylobacterium extorquens

  

Methanol

Succinate

Plasmid

Promoter

Uninduced

Induced

Uninduced

Induced

pJP18T

P R/cmtO

5.32 ± 0.64

28.94 ± 2.14

5.79 ± 0.44

61.93 ± 2.42

pJP22T

P R/tetO

1.46 ± 0.27

33.13 ± 2.63

1.95 ± 0.62

54.67 ± 5.60

  1. Gene expression as measured by mCherry fluorescence from M. extorquens cells harboring pJP18T or pJP22T. Cells were grown in succinate or methanol medium in the presence or absence of Q (5 µg/ml) or aTc (25 ng/ml). Values are relative fluorescence (arbitrary units) and reported error is the 95% confidence interval (N = 4).