|
Gene symbol
|
Forward primer sequence (5’- 3’)
|
Reverse primer sequence (5’- 3’)
|
Amplicon length (bp)
|
Primer location*
|
Efficiency (%)
|
|---|
|
GmCYP2
|
CGGGACCAGTGTGCTTCTTCA
|
CCCCTCCACTACAAAGGCTCG
|
154
|
S
|
98,3
|
|
GmELF1A
|
GACCTTCTTCGTTTCTCGCA
|
CGAACCTCTCAATCACACGC
|
195
|
D
|
102,4
|
|
GmTUA5
|
AGGTCGGAAACTCCTGCTGG
|
AAGGTGTTGAAGGCGTCGTG
|
159
|
S
|
101,7
|
|
GmELF1B
|
GTTGAAAAGCCAGGGGACA
|
TCTTACCCCTTGAGCGTGG
|
118
|
D
|
92,5
|
|
GmACT11
|
CGGTGGTTCTATCTTGGCATC
|
GTCTTTCGCTTCAATAACCCTA
|
142
|
D
|
104,1
|
|
GmUBC2
|
TCCCCTCACACCCTTCCTC
|
CCATCCCAAGGGGTGTCAT
|
155
|
D
|
107,6
|
|
GmTUB
|
CCTCGTTCGAATTCGCTTTTTG
|
CAACTGTCTTGTCGCTTGGCAT
|
161
|
S
|
96,4
|
|
GmG6PD
|
ACTCCTTGATACCGTTGTCCAT
|
GTTTGTTATCCGCCTACAGCCT
|
126
|
D
|
110,7
|
|
GmUBC4
|
GAGCGAGCAGTTTCAGAC
|
CATAGGAGGGACGATACG
|
168
|
D
|
98
|
|
GmRB7
|
TTGTAGGTGTCTCCGTCGC
|
AATGCTCTTGGCGGTGATG
|
179
|
S
|
87,3
|
- *Single exon (S) and different exons (D).
