Validation of reference genes aiming accurate normalization of qPCR data in soybean upon nematode parasitism and insect attack

Vívian de Jesus Miranda; Roberta Ramos Coelho; Antônio Américo Barbosa Viana; Osmundo Brilhante de Oliveira Neto; Regina Maria Dechechi Gomes Carneiro; Thales Lima Rocha; Maria Fatima Grossi de Sa; Rodrigo Rocha Fragoso

doi:10.1186/1756-0500-6-196

Table 2 Expression stability values and rankings of the reference genes calculated by NormFinder software

From: Validation of reference genes aiming accurate normalization of qPCR data in soybean upon nematode parasitism and insect attack

A
Organs V4		Organs R2		Organs R4		Root - development		Stem - development
Ranking	Stabilityvalue	Ranking	Stability value	Ranking	Stability value	Ranking	Stability value	Ranking	Stability value
GmELF1A	0,189	GmACT11	0,187	GmCYP2	0,169	GmELF1A	0,189	GmCYP2	0,335
GmUBC2	0,321	GmTUA5	0,451	GmELF1A	0,180	GmUBC2	0,190	GmTUA5	0,386
GmACT11	0,429	GmELF1A	0,499	GmACT11	0,188	GmCYP2	0,306	GmUBC2	0,430
GmUBC4	0,535	GmG6PD	0,549	GmTUA5	0,217	GmUBC4	0,331	GmACT11	0,490
GmTUA5	0,593	GmUBC4	0,617	GmUBC4	0,385	GmTUA5	0,390	GmELF1A	0,672
GmELF1B	0,605	GmELF1B	0,692	GmELF1B	0,396	GmELF1B	0,557	GmG6PD	0,767
GmG6PD	0,896	GmUBC2	0,703	GmUBC2	0,450	GmG6PD	0,742	GmUBC4	0,792
GmCYP2	1,046	GmCYP2	0,738	GmG6PD	0,980	GmACT11	0,899	GmELF1B	0,932
GmTUB	2,966	GmTUB	4,138	GmTUB	2,095	GmTUB	1,393	GmTUB	3,529
Best combination	Stability value	Best combination	Stability value	Best combination	Stability value	Best combination	Stability value	Best combination	Stability value
GmELF1A	0,203	GmACT11	0,250	GmCYP2	0,130	GmELF1A	0,167	GmCYP2	0,259
and GmUBC2		and GmTUA5		and GmELF1A		and GmUBC2		and GmTUA5
B
Leaf - development		Spatial/Temporal		*M. incognita* -inoculated root		*A. gemmatalis* -infested leaf		Total
Ranking	Stability value	Ranking	Stability value	Ranking	Stability value	Ranking	Stability value	Ranking	Stability value
GmELF1A	0,183	GmACT11	0,298	GmELF1A	0,1333	GmCYP2	0,060	GmACT11	0,089
GmACT11	0,230	GmTUA5	0,349	GmACT11	0,1660	GmELF1A	0,067	GmELF1A	0,124
GmUBC2	0,412	GmUBC2	0,448	GmTUA5	0,2221	GmACT11	0,081	GmTUA5	0,236
GmCYP2	0,433	GmELF1A	0,493	GmG6PD	0,3143	GmG6PD	0,087	GmG6PD	0,348
GmELF1B	0,451	GmG6PD	0,527	GmUBC2	0,3177	GmELF1B	0,121	GmELF1B	0,425
GmTUA5	0,545	GmCYP2	0,669	GmTUB	0,3267	GmTUA5	0,145	GmCYP2	0,559
GmUBC4	0,670	GmELF1B	0,722	GmCYP2	0,3951	GmUBC4	0,222	GmUBC4	0,621
GmG6PD	0,925	GmUBC4	0,748	GmELF1B	0,4186	GmTUB	0,293	GmUBC2	0,647
GmTUB	1,219	GmTUB	2,638	GmUBC4	0,7903	GmUBC2	0,948	GmTUB	1,246
Best combination	Stability value	Best combination	Stability value	Best combination	Stability value	Best combination	Stability value	Best combination	Stability value
GmELF1B and GmTUA5	0,177	GmACT11 and GmTUA5	0,176	GmACT11 and GmELF1A	0,133	GmCYP2 and GmELF1A	0,057	GmACT11 and GmELF1A	0,084

Stability values are listed from the most stable genes to the least stable.

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