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Table 2 Expression stability values and rankings of the reference genes calculated by NormFinder software

From: Validation of reference genes aiming accurate normalization of qPCR data in soybean upon nematode parasitism and insect attack

A          
Organs V4   Organs R2   Organs R4   Root - development Stem - development
Ranking Stabilityvalue Ranking Stability value Ranking Stability value Ranking Stability value Ranking Stability value
GmELF1A 0,189 GmACT11 0,187 GmCYP2 0,169 GmELF1A 0,189 GmCYP2 0,335
GmUBC2 0,321 GmTUA5 0,451 GmELF1A 0,180 GmUBC2 0,190 GmTUA5 0,386
GmACT11 0,429 GmELF1A 0,499 GmACT11 0,188 GmCYP2 0,306 GmUBC2 0,430
GmUBC4 0,535 GmG6PD 0,549 GmTUA5 0,217 GmUBC4 0,331 GmACT11 0,490
GmTUA5 0,593 GmUBC4 0,617 GmUBC4 0,385 GmTUA5 0,390 GmELF1A 0,672
GmELF1B 0,605 GmELF1B 0,692 GmELF1B 0,396 GmELF1B 0,557 GmG6PD 0,767
GmG6PD 0,896 GmUBC2 0,703 GmUBC2 0,450 GmG6PD 0,742 GmUBC4 0,792
GmCYP2 1,046 GmCYP2 0,738 GmG6PD 0,980 GmACT11 0,899 GmELF1B 0,932
GmTUB 2,966 GmTUB 4,138 GmTUB 2,095 GmTUB 1,393 GmTUB 3,529
Best combination Stability value Best combination Stability value Best combination Stability value Best combination Stability value Best combination Stability value
GmELF1A 0,203 GmACT11 0,250 GmCYP2 0,130 GmELF1A 0,167 GmCYP2 0,259
and GmUBC2   and GmTUA5   and GmELF1A   and GmUBC2   and GmTUA5  
B          
Leaf - development Spatial/Temporal M. incognita -inoculated root A. gemmatalis -infested leaf Total
Ranking Stability value Ranking Stability value Ranking Stability value Ranking Stability value Ranking Stability value
GmELF1A 0,183 GmACT11 0,298 GmELF1A 0,1333 GmCYP2 0,060 GmACT11 0,089
GmACT11 0,230 GmTUA5 0,349 GmACT11 0,1660 GmELF1A 0,067 GmELF1A 0,124
GmUBC2 0,412 GmUBC2 0,448 GmTUA5 0,2221 GmACT11 0,081 GmTUA5 0,236
GmCYP2 0,433 GmELF1A 0,493 GmG6PD 0,3143 GmG6PD 0,087 GmG6PD 0,348
GmELF1B 0,451 GmG6PD 0,527 GmUBC2 0,3177 GmELF1B 0,121 GmELF1B 0,425
GmTUA5 0,545 GmCYP2 0,669 GmTUB 0,3267 GmTUA5 0,145 GmCYP2 0,559
GmUBC4 0,670 GmELF1B 0,722 GmCYP2 0,3951 GmUBC4 0,222 GmUBC4 0,621
GmG6PD 0,925 GmUBC4 0,748 GmELF1B 0,4186 GmTUB 0,293 GmUBC2 0,647
GmTUB 1,219 GmTUB 2,638 GmUBC4 0,7903 GmUBC2 0,948 GmTUB 1,246
Best combination Stability value Best combination Stability value Best combination Stability value Best combination Stability value Best combination Stability value
GmELF1B and GmTUA5 0,177 GmACT11 and GmTUA5 0,176 GmACT11 and GmELF1A 0,133 GmCYP2 and GmELF1A 0,057 GmACT11 and GmELF1A 0,084
  1. Stability values are listed from the most stable genes to the least stable.
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