Figure 2From: PCR on yeast colonies: an improved method for glyco-engineered Saccharomyces cerevisiaeLyticase treatment of the YiMMOgène colonies can improve PCR efficiency. A) OD800 measurement during lyticase treatment, 2 independent assays. OD800 was measured on an Ultrospec 2100pro (Amersham). B) Percentage of MNN5 amplification from 20 colonies picked on Petri dishes treated or untreated with lyticase for 2 h.Back to article page