Development of a proof of concept immunochromatographic lateral flow assay for point of care diagnosis of Mycobacterium tuberculosis

Table 1 Percent antibody positive responders by ELISA in different population groups to candidate antigens

Antigen	Healthy controls (Denmark, n = 79)	TB patients (Denmark, n = 119)	TB patients (Uganda, n = 77)	Healthy controls (Ethiopia, n = 58)	TB patients (Ethiopia n = 79)
ESAT-6: CFP10	3	45	91	31	74
TB16.3	4	38	56	ND	ND
MPT83: Acry	4	72	74	19	64
CYP15	4	10	30	17	72
CFP21	8	35	50	34	46
Rv1575	18	28	67	50	62
ORF3	5	15	55	16	71
CFP8A	6	38	74	28	53
Rv3118	15	28	72	ND	ND
Rv0222	3	74	89	25	78

A positive result was defined as the negative control (serum without antigen), plus 3 standard deviations as previously described [18]. Those antigens selected for further development in the LFA are written in bold.

ISSN: 1756-0500