Figure 1
From: Domain analysis of symbionts and hosts (DASH) in a genome-wide survey of pathogenic human viruses
DASH’s computational pipeline. DASH compares a pathogen genome against the HMM profiles in Pfam using HMMER. As illustrated in the left-hand example, DASH compares all pathogen proteins against all PfamA profiles, annotating the domain function on the sequences and assigning E-value scores for the comparisons. All similarities with E-values under the user-defined threshold are reported as functional annotations in the pathogen genome. Likewise, DASH functionally annotates the host genome in parallel. With fully annotated genomes, DASH can distinguish the protein functions exclusive to the pathogen, functions exclusive to the host, and functions shared by both organisms.