Western blot assayof CspA-FLAG, CspB-FLAG and the graphical presentation. Western blot assay during the periodic temperature cycling (A) and during the cold shock (B) is shown. The relative amounts of CspA-FLAG (empty squares), CspB-FLAG (full squares), the relative total protein concentration in CspA-Flag cells (empty triangles), and the relative total protein concentration in CspB-FLAG cells (crosses) during the periodic temperature cycling (C) and during the cold shock (D) are shown. The amounts of proteins are plotted relative to the level prior to the first temperature down shift, whereas protein values during the cold shock are normalized to the protein values just before temperature downshift. The data were obtained from three independent experiments. Mean values and standard deviation are given.