Cold shock CspA and CspB protein production during periodic temperature cycling in Escherichia coli

BMC Research Notes

Table 1 Protein identification

General function	Spot number	Protein name or function	NCBInr accession no.	Gene	Up or down regulation		MOWSE score	Sequence coverage (%)
					temperature cycled cells relative to cells constantly at 37°C	cold shock cells relative to cells constantly at 37°C
Stress response	1	Cold shock-like protein cspB	gi\|16129516	cspB	de novo	de novo	301	52
	2	Cold shock protein cspA	gi\|15804102	cspA	+ 5.1	+ 2.8	310	52
Energy metabolism	3	Succinyl-CoA synthetase subunit alpha	gi\|1580008	sucD	+ 2.7	NC	105	5
Peptide transport	4	Periplasmic oligopeptide-binding protein	gi\|1583099	oppA	+ 3.1	NC	340	11
Sugar transport	5	Maltose-binding periplasmic protein	gi\|15834271	malE	+ 5.8	NC	519	17
Ion transport	6	Outer membrane porin protein nmpC	gi\|145856	nmpC	- 10.0	NC	555	26
Electron transport	7	Flavodoxin-1	gi\|2781032	fldA	- 2.1	NC	532	38
Amino acid biosynthesis	8	Phosphoserine aminotransferase	gi\|3891552	serC	- 2.4	NC	260	14
Protein synthesis	9	30S ribosomal protein S16	gi\|15803131	rpsP	NC	+ 3.6	96	19
	10	50S ribosomal protein L27	gi\|15833318	rpmA	NC	+ 2.5	112	16

Proteins that were significantly (p < 0.05) up (+) or down (−) regulated during the periodic temperature cycling or the cold shock relative to the protein levels incubated constantly at 37°C. NC (not changed).

ISSN: 1756-0500