Rapid assay to assess colonization patterns following in-vivo probiotic ingestion

BMC Research Notes

Table 1 PCR primer and probes sequences utilized in the study

	Sequence (5′-3′)^a
Target	Primer/probe	Ref
B. animalis subsp. lactis	F: GTGGAGACACGGTTTCCC	[13]
	R: CACACCACACAATCCAATAC	[13]
	P: FAM-TTCACAGGTGGTGCATGGTCGT BHQ1	This paper
B. longum subsp. infantis	F: TTCCAGTTGATCGCATGGTC	[12]
	R: GGAAACCCCATCTCTGGGAT	[12]
	P: CY5-TCAAgCCCAggTAAggTTCTTCgC BHQ3	This paper
S. thermophilus	F: TTATTTGAAAGGGGCAATTGCT	[11] Modified
	R: GTGAACTTTCCACTCTCACAC	[11] Modified
	P: CY5-ACTACAAGATGGACCTGCGT BHQ3	This paper

^aNucleotide bases among the primers and probes that are underlined represent locked nucleic acid bases. F, forward primer; R, reverse primer; P, probe; FAM, 6-carboxyfluorescein; Cy5, cyanine 5; BHQ, Black Hole Quencher.

ISSN: 1756-0500