Figure 4

Soybean cyst nematode development in soybean roots silenced for SHMT using virus-induced gene silencing. EXF67 and EXF63 are SCN-resistant and SCN-susceptible RILS, respectively. Ten days after planting, soybean leaves were rub-inoculated with either BPMV (Bean pod mottle virus) or BPMV containing a fragment of the SHMT gene sequence (BPMV-SHMT). Diamonds represent the number of cysts on a single root system at 35 days post-inoculation. At least twelve plants per genotype treatment were used. Cysts were collected on a 250 μM sieve and counted manually under a dissecting microscope. Six independent experiments were performed showing similar results. Data from one experiment are presented. Different letters denote a significant difference at P <0.0001. (B) Effect of BPMV-SHMT on SHMT transcript levels in soybean roots of ExF67. Reprinted from reference [23] with permission from Nature.