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Table 1 Primer sequences

From: Control and target gene selection for studies on UV-induced genotoxicity in whales

Primer

Sequence 5′- 3′

GC

Tm

Size

Eff

R2

S18-F

CAATTAAGGGTGTGGGGCGAAG*

54.5

62.1

141

99.0

1.000

S18-R

TCTTGTATTGGCGTGGATTCTGC*

47.8

60.6

SDHA-F

TGTTTCCCACCAGGTCACACAC*

54.5

62.1

119

93.4

0.991

SDHA-R

CCAGTCGGAGCCCTTCACG*

68.4

63.1

PGK1-F

ACAATGGAGCCAAGTCAG*

50.0

53.7

146

91.9

0.998

PGK1-R

CACGCAGTCCTTCAAGAAC*

52.6

56.7

RPL4-F

CAGACCTTAGCAGAATCTTGAAAAGC*

42.3

61.6

171

92.0

0.998

RPL4-R

CCTGGCGAAGAATGGTGTTCC*

57.1

61.8

HSP70-F

GTCAAGCACGGTGTTCTGTG

55.0

59.4

141

101.2

0.999

HSP70-R

CACGGCAAAGTAGAGATCATCG

50.0

60.3

P53-F

CTCACCATCATCACACTGGA

50.0

57.3

175

94.2

0.998

P53-R

TAGGCAGTGCTCGCTTAGC

57.9

58.8

KIN-F

TGCTGGCTTCAGAAAATCC

47.4

54.5

98

92.3

0.997

KIN-R

CTCTTGGTTCCAAAGCGTCTC

52.4

59.8

  1. F = forward; R = reverse; GC = GC content (percentage); Tm corresponds to the theoretical melting temperature. R2 corresponds to the linear correlation coefficient of the standard curve obtained by plotting the logarithm of the quantity of gene expression versus the threshold cycle (Ct). The slope of the curve was used to calculate the amplification efficiency (in%) for each pair of primers (Eff = (101/-slope-1)*100). * Primers tested previously in dolphins [13].