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Figure 3 | BMC Research Notes

Figure 3

From: Reverse transcription quantitative real-time polymerase chain reaction reference genes in the spared nerve injury model of neuropathic pain: validation and literature search

Figure 3

Determination of the optimal number of control genes for accurate normalization. Determination of the number of reference genes to consider for an accurate normalization in spinal cord dorsal horn (A) and dorsal root ganglia (B) by calculating pairwise variation (V) after stepwise elimination of the worst reference gene.

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