Reverse transcription quantitative real-time polymerase chain reaction reference genes in the spared nerve injury model of neuropathic pain: validation and literature search

Table 1 Specification of used reference genes

Gene symbol	Sequence (5'->3')	Position	Product length	Intron inclusion	RTqPCR efficiency in DH/DRG	Ref.
Full name	Sequence (5'->3')	Position	Product length	Intron inclusion	RTqPCR efficiency in DH/DRG	Ref.
Accession number
18S	Fw: GGCTCATTAAATCAGTTATGGTTCCT	94-119	147	no	78%/70%	[22]
18S ribosomal RNA	Rev: GTTGGTTTTGATCTGATAAATGCACG	240-215
V01270	Rev: GTTGGTTTTGATCTGATAAATGCACG
RPL29	Fw: ACAGAAATGGCATCAAGAAACCC	96-118	105	yes	81%/77%	[15]
Ribosomal protein L29	Rev: TCTTGTTGTGCTTCTTGGCAAA	200-179	105	yes	81%/77%	[15]
NM_017150
RPL13a	Fw: TCTCCGAAAGCGGATGAACAC	185-205	145	yes	88%/73%	[14]
Ribosomal protein L13A	Rev: CAACACCTTGAGGCGTTCCA	329-310	145	yes	88%/73%	[14]
NM_173340
Actb	Fw: GGAGATTACTGCCCTGGCTCCTA	1023-1045	150	yes	85%/63%	[14]
Actin, beta	Rev: GACTCATCGTACTCCTGCTTGCTG	1172-1149
NM_031144	Rev: GACTCATCGTACTCCTGCTTGCTG	1172-1149
HPRT1	Fw: GCATCTAAGAGGTTTCCCCAGT	1133-1154	76	no	70%/74%	¹
Hypoxanthine phosphoribosyl-transferase 1	Rev: GCATTTAAAAGGAACGGTTGAC	1208-1187	76	no	70%/74%	¹
NM_012583
HMBS	Fw: GAGACCATGCAGGCCACCAT	1007-1026	97	yes	78%/72%	[15]
Hydroxymethyl-bilane synthase	Rev: TTGGAATGTTCCGGGCAGTG	1084-1103
NM_013168	Rev: TTGGAATGTTCCGGGCAGTG	1084-1103
GAPDH	Fw: CCCCCAATGTATCCGTTGTG	780-799	118	yes	89%/87%	[23]
Glyceraldehyde-3-phosphate	Rev: TAGCCCAGGATGCCCTTTAGT	877-897	118	yes	89%/87%	[23]
dehydrogenase
NM_017008

Fw forward, Rev reverse primer sequence, DH dorsal horn, DRG dorsal root ganglion.
¹ from our laboratory database.

ISSN: 1756-0500