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Table 2 List of reference genes in the rat spared nerve injury model

From: Reverse transcription quantitative real-time polymerase chain reaction reference genes in the spared nerve injury model of neuropathic pain: validation and literature search

Article

PCR method

Sample

Reference genes

Validation

Kanda et al., 2013[25]

PCR

L4/L5 SC

GAPDH

no

Zhou et al., 2013[26]

RT-qPCR

L4/L5 DRG

GAPDH

no

Kashimoto et al., 2013[27]

RT-qPCR

L4/L5 SC

GAPDH

no

Shankarappa et al., 2012[28]

RT2 Profiler PCR array

L4/L5 DRG

HPRT1

validated1

Inquimbert et al., 2012[29]

PCR

L4/L5 DH

GAPDH

no

Zapata et al., 2012[30]

RT-qPCR

RVM

18S and HMBS

no

Samad et al., 2013[31]

PCR

L4 DRG

GAPDH

no

Kobayashi et al., 2012[32]

RT-qPCR

L4/L5 SC

GAPDH

no

Liu et al., 2012[33]

RT-qPCR

L4–L6 SC

Actb

no

Tochiki et al., 2012[34]

PCR

L4-L6 DH

HPRT1

no

Okubo et al., 2012[35]

RT-qPCR

L4-L5 SC

GAPDH

no

Del Rey et al., 2011[36]

PCR

hippocampus

not mentioned

no

Kühlein et al., 2011[37]

RT-qPCR and PCR

DH and DRG

Actb and 18S

no

Yamanaka et al., 2011[38]

RT-qPCR

L4, 5 DRG

GAPDH

no

de Novellis et al., 2011[39]

RT-qPCR

mPFC

Actb

no

Vega-Avelaira et al., 2009[40]

PCR

L4/L5 DRG

GAPDH

no

Costigan et al., 2009[41]

PCR

L4/L5 DH

GAPDH

no

Okubo et al., 2010[42]

RT-qPCR

L4-L5 SC

GAPDH

no

Staaf et al., 2009[43]

Taqman Low Density Arrays

L4 DRG

18S

validated2

Moss et al., 2008[44]

PCR

L4/5 DRG

GAPDH

no

Berta et al., 2008[45]

PCR

L4 and L5 DRGs

GAPDH

no

Millecamps et al., 2007[46]

PCR

mPFC

GAPDH

no

Moss et al., 2007[47]

PCR

L4/L5 dorsal horn

GAPDH

no

Apkarian et al., 2006[48]

RT-qPCR

brainstem, thalamus, and prefrontal cortex

GAPDH

no

Pertin et al., 2005[49]

PCR

L4/L5 DRG

GAPDH

no

Takahashi et al., 2003[50]

RT-qPCR

L5 DRG

GAPDH

no

  1. Literature search in the PubMed database of studies using semi-quantitative PCR or RT-qPCR in the rat SNI model.
  2. PCR semi-quantitative polymerase chain reaction, RT-qPCR reverse transcription quantitative real-time polymerase chain reaction, SC spinal cord, DRG dorsal root ganglion, DH dorsal horn, RVM rostral ventromedial medulla, PL-IL prelimbic and infralimbic, mPFC medial prefrontal cortex.
  3. 1 HPRT1 validated among 5 reference genes.
  4. 2 See Discussion.