Figure 3

Promoter activity determined in M. tuberculosis . (A) Upstream regions of GyrB ending in Met and Val were cloned into pSM128 to give pSM128-Met and pSM128-Val respectively. (B) M. tuberculosis recombinants were grown as described in Materials and Methods, and β-galactosidase activity measured. Transformants were obtained for pSM128 (empty vector control); pSM128-Val (containing M. tuberculosis nucleotides 4998–5125, upstream of the ORF for GyrB) and pSM128-Met (containing nucleotides 4998–5242). Data are mean +/- standard deviation from three independent transformants tested in duplicate. (C) M. tuberculosis genome sequence 4981–5280 that includes the start of gyrB and its upstream sequence. The predicted ribosome binding site is in bold and underlined; the predicted promoter elements are in bold; the gyrB coding sequence is in italics with the Val start codon underlined. The annotations are consistent with earlier work [10].