Figure 3

Quercetin reduced leptin-induced ERK1/2 phosphorylation. (A) Western blot analysis of phosphorylated ERK1/2 in HUVECs showed that 500 ng/mL leptin exposure for 48 h increased ERK1/2 phosphorylation (without (lane 1) and with (lane 3) leptin exposure). Quercetin alone did not show any effect (lane 2), nevertheless in leptin-induced HUVECs, ERK1/2 phosphorylation was reduced (lane 4). (B) Total phosphorylated ERK1/2 level measured by ELISA was reduced after quercetin treatment on leptin-induced HUVECs. L0Q0 control HUVECs without leptin and quercetin; L0Q125 without leptin, but with 125 μM quercetin; L500Q0 with 500 ng/mL leptin, but without quercetin; L500Q125 with 500 ng/mL leptin and 125 μM quercetin. Means without a common letter differ, at least p < 0.05.