Figure 3
14C-3-BrPA primarily binds with two peptides in rat serum. (A) A Coomassie stained 2D-gel showing serum protein spots after 120 minutes of 3-BrPA treatment and (B) a corresponding autoradiogram showing a strong and also a weak signal at ~50-60 kDa. (C) and (D) LC-MS/MS base-peak chromatograms of the peptide spots corresponding to strong (*) and weak signals on 2D-autoradiogram with the list of possible peptides, in the order of matched score.