Figure 1From: Differences in PAR-2 activating potential by king crab (Paralithodes camtschaticus), salmon (Salmo salar), and bovine (Bos taurus) trypsinPurified king crab trypsin differs in size from purified sardine trypsin. 250 ng of purified king crab and sardine trypsin were run on a SDS-PAGE gel and stained with silver staining for detection. The result shows that purified king crab trypsin (lane 7 and 8) is a slightly bigger molecule residing in the 28 – 30 kDa area compared to the purified sardine trypsin (lane 3 and 4) at 24 – 25 kDa. Additional purified fish trypsins tested (anchovy (lane 5 and 6), yellow tail, jacopever, spotted mackerel (not shown)) displayed similar size as purified sardine trypsin. Lane 1, 2 and 10 contain protein standards, respectively 10 μl SeeBlue®,10 μl Mark 12™, 16 μl SeeBlue®. Lane 9 is empty.Back to article page