Table 4 Oligonucleotides used for strain construction
From: Role of the Candida albicans MNN1 gene family in cell wall structure and virulence
Primer | Sequence (5′-3′)a |
|---|---|
MNN1-5H | AAGCTTtctacagtggctgttaataatg |
MNN1-5S | GCATGCgaaaactcatacaattcactaag |
MNN1-3A | GGTACCtgcttactcaagtgttggtg |
MNN1-3B | GAGCTCgttagtggcttgtatcaaagg |
MNN12-5H | AAGCTTatcataacacgttcgttcagc |
MNN12-5S | GCATGCtgacagatcatcgtcttcatc |
MNN12-3A | GGTACCatgccattattccaccaatgg |
MNN12-3B | GAGCTCgttgtgactgtggctccac |
MNN13-KO-F | gttgactttgaagagccactgtatactttccctaagacggtgctatttgaaaacaacaacaatttggatgatttattatGCTCGGATCCACTAGTAACG |
MNN13-KO-R | ttaatattttccaacccatacatctcctaagtaattgaaatatgcaatttcttcaggggtatactcaaccaataaaccaCCAGTGTGATGGATATCTGC |
MNN14-KO-F | atgcattttatttggcaatcacaagccaattctggattaatactaaaaaatgaattatcactgacatcaagtatcccccGCTCGGATCCACTAGTAACG |
MNN14-KO-R | ctataaataattatatcccctcatccatatctccccaagctttttaaaatgttctatttcctctggtgaataattaattCCAGTGTGATGGATATCTGC |
MNN15-KO-F | cgtataagggaacttttacatgatagtgaaaaatttgatctcgagagtctaaagcaagctagcttggagataagaaaagGCTCGGATCCACTAGTAACG |
MNN15-KO-R | ctttatccgaaccagttccaagtttctcagtttctaatttaacttttggcttcattttggatccaccagtgtgccatatCCAGTGTGATGGATATCTGC |
MNN16-KO-F | gaagttccatcttaaaagatatgtcatagttacttcaatactactatcttttttcttattattcagacgacaatttcttGCTCGGATCCACTAGTAACG |
MNN16-KO-R | ttaatcttgtaaccaaatttcaattaatttcttatattttttttgtaaactttgttcaacattaatagataatcctctaCCAGTGTGATGGATATCTGC |