Figure 3

Synthetic sequence evolved to stabilize the PDZ domain. (A) Snapshot of the results from e Volver webserver, which shows the final fitness score, the SA trajectory and the evolved sequence in FASTA format. (B) Output from PSI-BLAST obtained by using the evolved sequence to query PDB. (C) Structure alignments of the top 3 PSI-BLAST hits (3k82A – yellow, 3i4wA – green, 1tp3A – blue) against the target structure (2omjA – red); aligned regions are solid.