Table 8 Primers used for real-time PCR expression analysis of six candidate reference genes
Name | Forward primer 5′-3′ | Reverse primer 5′-3′ | Length (bp) | Tm (°C) |
|---|---|---|---|---|
18S rRNA | CGCGCGCTACACTGATGTATTCAA | TACAAAGGGCAGGGACGTAGTCAA | 172 | 60 |
APRT | GAGATGCATGTAGGTGCTGTGCAA | GGCCCTTCAATTCTGGCAACTCAA | 163 | 60 |
GAPDH | ATGGCCTTCAGAGTACCAACTGCT | GCTTGACCTGCTGTCACCAACAAA | 189 | 60 |
Cyclophilin | GCGCCAAATTCAAGGACGAGAACT | ACAGCCTCGGCCTTCTTAATCACA | 196 | 60 |
Actin | TGTGTTGGACTCTGGTGATGGTGT | TCACATCCCTGACGATTTCTCGCT | 185 | 60 |
RuBP | TCGAGACTGAGCACGGATTTGTGT | TGCACTCTCCGACCTCATTCAACA | 141 | 60 |