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Table 3 Overall comparison of cold stain method with the Ziehl-Neelsen method

From: Comparative evaluation of a two-reagent cold stain method with Ziehl-Nelseen method for pulmonary tuberculosis diagnosis

ZN; no. (%)

Report

Positive

Negative

Total

Positive CS

23(6.4)

0(0)

23(6.4)

Negative

1(0.3)

338(93.4)

339(93.6)

Total

24(6.6)

338(93.4)

362(100)

  1. ZN Ziehl-Neelsen, CS Cold stain; Positive = any positive (scanty, 1+, 2+ and 3+); Negative = no Acid fast bacilli per oil immersion in at least 100 fields; Sensitivity = 95.8%; Specificity = 100%; Positive predictive value =100%; Negative predictive value = 99.7%; Agreement = 99.7; Kappa (κ) = 0.98.