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Table 4 Comparison of ZN and CS methods for detection and grading of AFB in first-spot sputum specimens

From: Comparative evaluation of a two-reagent cold stain method with Ziehl-Nelseen method for pulmonary tuberculosis diagnosis

ZN; no. (%)/24

 

Grade

3+

2+

1+

Scanty

Negative

Total

 

3+

5(1.4)

0(0)

0(0)

0(0)

0(0)

5(1.4)

 

2+

3(0.8)

8(2.2)

0(0)

0(0)

0(0)

11(3.0)

CS/23

1+

0(0)

0(0)

6(1.7)

0(0)

0(0)

6(1.7)

 

Scanty

0(0)

0(0)

0(0)

1(0.3)

0(0)

1(0.3)

 

Negative

0(0)

0(0)

0(0)

1(0.3)

338(93.4)

339(93.6

 

Total

8(2.2)

8(2.2)

6(1.7)

2(0.6)

338(93.4)

362(100)

  1. AFB acid fast bacilli; 3+ = More than 10 AFB per oil immersion field in at least 20 fields; 2+ = 1–9 AFB per oil immersion field in at least 50 fields; 1+ = 10–99 AFB in 100 oil immersion fields; Scanty = 1–9 AFB in 100 oil immersion fields.