Table 1 Characterization of nine microsatellite loci isolated from Atta laevigata
From: Microsatellite loci characterized in the leaf-cutter ant Atta laevigata
Locus | Primer sequence (5'-3', forward / reverse) | Annealing (°C) | Repeat motif | N | n a | Size (bp) | H o | H e | HW p- value |
|---|---|---|---|---|---|---|---|---|---|
Alae2 | AGTTTCTGCAATATTCGC / TCTGTGAGAGAGCAAGTGAG | 49.0 | (TC) 1(TCC) 2(TC) 9tt(TC) 4 | 25 | 8 | 82-98 | 0.96 | 0.81 | 0.4407 |
Alae5 | GCAAAGACATCGTAAAGTG / TGCAACCGTCTTGTATG | 52.0 | (TAA) 5 | 27 | 2 | 128-137 | 0.07 | 0.07 | 1.0000 |
Alae9 | TCTTGTAAGTAACTGTCGAGC / CGTCATATCCGAATGTCAG | 45.0 | (CT)t(CT)23 | 24 | 11 | 124-168 | 0.96 | 0.88 | 0.8732 |
Alae10 | CGCTACATCCCATCTCAC / GACAGCAATATTTCGATAGC | 44.0 | (GA) 19ca(GA) | 27 | 9 | 110-130 | 0.78 | 0.84 | 0.0745 |
Alae11 | CACGATAGTTTTCGATATCC / TGGGTGTATCAAAGAAAGAC | 45.0 | (GA) 15 | 26 | 10 | 100-128 | 0.58 | 0.65 | 0.3458 |
Alae16 | ACTATGTCCATGTTATGCG / GACTACAAGTAAGAATAGTGAGC | 56.0 | (TC)3tg(TC)3tg(TC)13tg(TC)3tt(TC)7 | 25 | 7 | 188 -212 | 0.72 | 0.87 | 0.0145 |
Alae18 | ACATGTCCACTCCGTCAG / CGATAGCGTGATATTTGC | 56.0 | (TG)11 | 22 | 16 | 144-188 | 0.32 | 0.94 | 0.0000* |
Alae19 | GACGTGGAGCTGCAATAC / AAGTGAGTACAAAACATACAGG | 50.0 | (CAAA)5 | 22 | 2 | 89-93 | 0.32 | 0.33 | 1.0000 |
Alae24 | GCAATAAATTCAGATGGC / CTGCAAAATCACAGTTGC | 52.8 | (CT)20 | 25 | 10 | 169-191 | 0.72 | 0.87 | 0.1904 |