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Figure 2 | BMC Research Notes

Figure 2

From: The impact of reference gene selection in quantification of gene expression levels in guinea pig cervical tissues and cells

Figure 2

Pairwise variation of reference gene candidates in cervical cells and tissues as determined by geNorm. The first bar (2/3) represents the pairwise variation (V) in a 2 vs. 3 reference genes comparison. The second bar (3/4) represents pairwise variations in a 3 vs. 4 reference gene comparison. Variation values were determined using from 2/3 up to 13/14 reference gene comparisons in cultured stromal cells (A), cultured epithelial cells (B), stromal tissues (C), and epithelial tissues (D). Pairwise variation values of ≤0.15 were considered sufficient for normalization, values above 0.15 are considered too variable. Note increased V values in tissues compared with cells in culture.

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