Figure 3

Effect of normalization options on PTGS2 gene expression in cultured cervical cells. PTGS2 expression was normalized to either the least stable (white), the top two most stable (gray) or the top three most stable (black) reference genes for each cell type as established with geNorm. Relative levels of PTGS2 mRNA were determined in (A) cervical epithelial cells and (B) cervical stromal cells treated with vehicle (C), estradiol (E, 10 nM), progesterone (P, 100 nM), or E+P. Error bars indicate 95% confidence interval; *, p<0.05; **, p<0.01; n=18.