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Table 2 Stability of various potential reference genes in guinea pig cervical tissues

From: The impact of reference gene selection in quantification of gene expression levels in guinea pig cervical tissues and cells

 

Stroma enriched

Epithelium enriched

 

geNorm

BestKeeper

geNorm

BestKeeper

Most stable

CFL1/CLTC (0.42)

CLTC (0.991)

CFL1/CLTC (0.29)

CLTC (0.995)

 

ACTB (0.48)

ATP5B/CFL1 (0.985)

PPIB (0.34)

CFL1 (0.991)

 

ATP5B (0.51)

B2M (0.983)

SDHA (0.38)

SDHA (0.983)

 

GAPDH (0.54)

ACTB (0.978)

GAPDH (0.41)

TPT1 (0.979)

 

PPIB (0.57)

TPT1 (0.977)

ATP5B (0.44)

B2M (0.970)

 

SDHA (0.62)

SDHA (0.975)

ACTB (0.47)

TFRC (0.966)

 

ATP6 (0.70)

RPLP0 (0.967)

HMBS (0.51)

PPIB (0.965)

 

RPLP0 (0.75)

ATP6 (0.955)

TFRC (0.55)

ACTB (0.958)

 

B2M (0.78)

CTBP1 (0.954)

ATP6 (0.57)

ATP5B (0.954)

 

TPT1 (0.83)

GAPDH (0.952)

B2M (0.61)

HMBS (0.950)

 

CTBP1 (0.89)

PPIB (0.937)

RPLP0 (0.65)

ATP6 (0.920)

   

CTBP1 (0.68)

GAPDH (0.92)

   

TPT1 (0.72)

CTBP1 (0.895)

Least stable

   

RPLP0 (0.892)

Excluded

HMBS

TBP

 

TBP

  
 

TFRC

  
  1. Genes listed with the most stable as determined by geNorm or BestKeeper on top. Genes excluded due to low expression levels in the respective tissue are listed at the bottom. The numbers in brackets represent geNorm M values and BestKeeper R values, respectively.