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Table 3 Stability of reference gene candidates in guinea pig cervical primary cell cultures

From: The impact of reference gene selection in quantification of gene expression levels in guinea pig cervical tissues and cells

  Stromal cells Epithelial cells
  geNorm BestKeeper geNorm BestKeeper
Most stable ACTB/PPIB (0.09) PPIB (0.999) CLTC/PPIB (0.10) CLTC (0.983)
  SDHA (0.12) ACTB (0.996) ATP5B (0.11) PPIB (0.981)
  B2M (0.15) SDHA (0.995) CTBP1 (0.12) ATP5B (0.975)
  CLTC (0.18) B2M (0.984) B2M (0.12) CFL1 (0.957)
  RPLP0 (0.20) CLTC (0.977) SDHA (0.13) CTBP1 (0.946)
  CTBP1 (0.21) GAPDH (0.974) HMBS (0.14) ATP6 (0.945
  GAPDH (0.23) RPLP0 (0.969) RPLP0 (0.15) TFRC (0.936)
  CFL1 (0.24) CFL1 (0.958) ATP6 (0.16) B2M (0.932)
  TPT1 (0.25) CTBP1 (0.957) TPT1 (0.18) ACTB (0.932)
  TFRC (0.28) TFRC (0.928) GAPDH (0.20) GAPDH (0.922)
  ATP5B (0.30) TPT1 (0.919) CFL1 (0.22) SDHA (0.867)
  ATP6 (0.33) ATP5B (0.908) ACTB (0.24) HMBS (0.842)
   ATP6 (0.857) TFRC (0.26) RPLP0 (0.838)
Least stable     TPT1 (0.602)
Excluded HMBS TBP
  TBP   
  1. Genes listed with the most stable as determined by geNorm or BestKeeper on top. Genes excluded due to low expression levels in the respective tissue are listed at the bottom. The numbers in brackets represent geNorm M values and BestKeeper R values, respectively.