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Table 3 Stability of reference gene candidates in guinea pig cervical primary cell cultures

From: The impact of reference gene selection in quantification of gene expression levels in guinea pig cervical tissues and cells

 

Stromal cells

Epithelial cells

 

geNorm

BestKeeper

geNorm

BestKeeper

Most stable

ACTB/PPIB (0.09)

PPIB (0.999)

CLTC/PPIB (0.10)

CLTC (0.983)

 

SDHA (0.12)

ACTB (0.996)

ATP5B (0.11)

PPIB (0.981)

 

B2M (0.15)

SDHA (0.995)

CTBP1 (0.12)

ATP5B (0.975)

 

CLTC (0.18)

B2M (0.984)

B2M (0.12)

CFL1 (0.957)

 

RPLP0 (0.20)

CLTC (0.977)

SDHA (0.13)

CTBP1 (0.946)

 

CTBP1 (0.21)

GAPDH (0.974)

HMBS (0.14)

ATP6 (0.945

 

GAPDH (0.23)

RPLP0 (0.969)

RPLP0 (0.15)

TFRC (0.936)

 

CFL1 (0.24)

CFL1 (0.958)

ATP6 (0.16)

B2M (0.932)

 

TPT1 (0.25)

CTBP1 (0.957)

TPT1 (0.18)

ACTB (0.932)

 

TFRC (0.28)

TFRC (0.928)

GAPDH (0.20)

GAPDH (0.922)

 

ATP5B (0.30)

TPT1 (0.919)

CFL1 (0.22)

SDHA (0.867)

 

ATP6 (0.33)

ATP5B (0.908)

ACTB (0.24)

HMBS (0.842)

  

ATP6 (0.857)

TFRC (0.26)

RPLP0 (0.838)

Least stable

   

TPT1 (0.602)

Excluded

HMBS

TBP

 

TBP

  
  1. Genes listed with the most stable as determined by geNorm or BestKeeper on top. Genes excluded due to low expression levels in the respective tissue are listed at the bottom. The numbers in brackets represent geNorm M values and BestKeeper R values, respectively.